High Resolution Dynamic Deuterium MRS Imaging of Cerebral Glucose Metabolism in Mouse Brain at Ultrahigh Field of 16.4T

Motivation: Motivation:Deuterium MRS imaging (2H-MRSI) is valuable for mapping brain deuterated metabolites, but its low sensitivity and poor imaging resolution make it particularly challenging to image the mouse brain. Goal(s): Goal:To study glucose metabolism in mouse brain using dynamic 2H-MRSI with high spatiotemporal resolution. Approach: Approach:Dynamic 3D 2H-MRSI was performed on mice brains at 16.4T with an intraperitoneal administration of deuterium labeled glucose; SPICE denoising was applied to further enhance SNR. Results: Results:We achieved highest spatiotemporal resolution 2H-MRSI (3μL voxel size and 2.5min) with excellent SNR, enabling imaging of low concentration metabolites and their dynamics in the mouse brain. Impact: This work demonstrates the capability of high-resolution 2H-MRSI for mapping deuterated metabolites and dynamics in the mouse brain with excellent sensitivity. It highlights the potential of studying cerebral glucose metabolism in various mouse models of genetic or other diseases.