A 384-Site Chip Platform for Biochemical Applications With Individual Site Precision Temperature Control

A chip platform for thermally controlled biochemistry is presented comprising postprocessed thermally isolated heated reaction sites atop a application-specific integrated circuit (ASIC) using proportional-integral-differential (PID) feedback temperature control. The 384 sites can be individually programmed over <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$25~^{\circ }$</tex-math> </inline-formula>C–<inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$125~^{\circ }$</tex-math> </inline-formula>C, with thermal crosstalk below the <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\pm 0.12~^{\circ }$</tex-math> </inline-formula>C precision. Double-strand deoxyribonucleic-acid (DNA) purification via selective melting is demonstrated, with 70% retention of perfectly matched sequences, while 55% of those with two mismatched base-pairs are removed.