Detection of highly pathogenic orthopoxviruses (<i>Poxviridae</i>: <i>Chordopoxvirinae</i>: <i>Orthopoxvirus</i>) by dot immunoassay in a high-containment biological safety laboratory
The one-step dot immunoassay can be performed in a BSL-4 laboratory. When limiting the contact of the detecting system with formaldehyde vapors, the sensitivity of the test for detection of VARV and MPXV falls within the previously declared range of 10<sup>3</sup>-10<sup>4</sup> PFU/mL.
Authors
- Pavel V. Filatov (ORCID: https://orcid.org/0000-0001-7763-3808)
- А. В. Ерш (ORCID: https://orcid.org/0000-0002-9220-1250)
- A. G. Poltavchenko (ORCID: https://orcid.org/0000-0003-2408-5611)
- Sergeev Aa (ORCID: https://orcid.org/0000-0002-3591-1571)
- Nikita D. Ushkalenko (ORCID: https://orcid.org/0000-0002-2171-7444)
- А. А. Сергеев (ORCID: https://orcid.org/0000-0001-8355-5551)
- К. А. Титова (ORCID: https://orcid.org/0000-0001-8764-9408)
- Dmitrii A. Odnoshevsky
Institutions
- State Research Center of Virology and Biotechnology VECTOR (RU)
Publication Details
- Journal
- Problems of Virology
- Published
- 2025-09-16
- DOI
- https://doi.org/10.36233/0507-4088-332
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 0.00