Labeling of Nascent RNA in C. elegans Intestine Cells v1

We present a novel protocol for metabolic labeling of nascent RNA in the C. elegans intestine, overcoming the challenge of RNA analogs' inability to penetrate the worm cuticle. This method involves dissecting worms to extrude their intestines and performing nascent RNA labeling in a tube. While optimized for imaging applications, the protocol can be adapted for other molecular techniques, such as sequencing or RT-qPCR, enabling quantitative analysis of nascent transcripts. This approach offers a powerful tool for investigating transcriptional regulation and RNA metabolism in the context of C. elegans physiology and disease models.