PHÂN LẬP, TUYỂN CHỌN VI KHUẨN PHÂN GIẢI CELLULOSE VÀ BƯỚC ĐẦU ỨNG DỤNG TRONG XỬ LÝ PHỤ PHẨM CÂY CHUỐI

In Vietnam, bananas occupy 19% of the total fruit cultivation area, yielding around 1.4 million tons but only 12% of the volume are utilized, the remaining 88% constitute organic waste. This research aimed to identify and select bacteria capable of breaking down cellulose to treating banana by-products. The Bacillus megaterium H3 strain from 12 soil and banana waste samples was isolated. Optimal conditions were determined for cultivating this bacterium to produce enzymes efficiently. The high-potency cellulase-producing Bacillus megaterium H3 bacteria was found to thrive in a medium with a CMC carbon source and NH4Cl nitrogen source. The most suitable culture conditions for this bacterium were at a temperature of 30°C, over a period of 16 hours, with a shaking speed of 200rpm, and an initial pH of 7. Additionally, supplementing banana stem waste with B. megaterium H3 facilitated 32.28% more efficient cellulose decomposition compared to the control sample. These findings serve as basis for the development of organic fertilizer.