Modified Common Fungal DNA Extraction Buffer (XNA) v2

This is a modified version of an extraction buffer that is widely used for fungal DNA extraction. It works very well. The high pH and EDTA will protect DNA from nuclease activity, even at room temperature for long periods of time. It is inexpensive and uses common reagents to make. A short example method is provided. Here is another protocol that is used by the Fungal Diversity Survey. dx.doi.org/10.17504/protocols.io.e6nvw1y59lmk/v2

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Publication Details

Published
2025-05-15
DOI
https://doi.org/10.17504/protocols.io.14egnyq8pv5d/v2
Primary Topic
Plant Pathogens and Fungal Diseases
Type
article
Field-Weighted Citation Impact
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article

Modified Common Fungal DNA Extraction Buffer (XNA) v2

Harte Singer, Stephen Douglas Russell
Plant Pathogens and Fungal Diseases
article

Modified Common Fungal DNA Extraction Buffer (XNA) v2

Harte Singer, Stephen Douglas Russell
article en

Abstract

This is a modified version of an extraction buffer that is widely used for fungal DNA extraction. It works very well. The high pH and EDTA will protect DNA from nuclease activity, even at room temperature for long periods of time. It is inexpensive and uses common reagents to make. A short example method is provided. Here is another protocol that is used by the Fungal Diversity Survey. dx.doi.org/10.17504/protocols.io.e6nvw1y59lmk/v2

Openalex Percentile: Top 74%
Plant Pathogens and Fungal Diseases
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Modified Common Fungal DNA Extraction Buffer (XNA) v2 — Harte Singer, Stephen Douglas Russell · (2025) | TGRS Research Map | TGRS