Harnessing conserved G-quadruplexes for fluorescence detection of <i>Mycobacterium tuberculosis</i>
We report the fluorescence detection of the conserved G-quadruplex (GQ) motif in <i>Mycobacterium tuberculosis</i> (MTB) DNA, ensuring high specificity with minimal interference from human and non-MTB DNA sequences. The duplex-to-GQ reliable conformational polymorphism (GQ-RCP) enables its application in amplification-compatible MTB detection, offering a platform for precise diagnostic assays.
Authors
- Mohamed Nabeel Mattath (ORCID: https://orcid.org/0000-0002-7309-7996)
- Sumon Pratihar (ORCID: https://orcid.org/0009-0008-9353-6125)
- Thimmaiah Govindaraju (ORCID: https://orcid.org/0000-0002-9423-4275)
- Krithi K. Bhagavath
- Vasudhar Bhat S V
Institutions
- Jawaharlal Nehru Centre for Advanced Scientific Research (IN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc04932f
- Citations
- 1
- Primary Topic
- DNA and Nucleic Acid Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.81