Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1

<strong>Objective:</strong> Phosphatase is primarily derived from soil microorganisms, and the composition of soil microbial communities greatly influences the availability of this enzyme source. Therefore, it is crucial to research soil bacteria capable of producing phosphatase. This study evaluated the phosphatase production ability of two bacterial strains, <em>Burkholderia vietnamiensis</em> 1.7 and<em> Priestia aryabhattai</em> 6.1, isolated from agricultural soil under different conditions. This study was conducted in vitro to assess the effects of shaking, pH level, salt conditions, and carbon and nitrogen nutrient sources on phosphatase production ability. <strong>Materials and Methods: </strong>Experiments were conducted under varying environmental conditions, including different shaking speeds (0, 60, 90, 120, and 150 rpm), medium pH levels (3, 5, 7, and 9), and NaCl concentrations (0, 1, 2, 3, 4, and 5%). The carbon sources used in the experiment were lactose, sucrose, maltose, and glucose, each at a concentration of 1%. The inorganic nitrogen sources were KNO₃, (NH₄)₂SO₄, (NH₄)₆Mo₇O₂₄&nbsp;, and CO(NH₂)₂ at all concentrations of 10%. Each experimental condition was tested in four replications. <strong>Results:</strong> <em>B. vietnamiensis</em> 1.7 and <em>P. aryabhattai</em> 6.1 exhibited the highest phosphatase production when cultured at a shaking speed of 150 rpm, pH 7, and a NaCl concentration of 1%, with glucose and urea as the carbon and nitrogen sources, respectively. <strong>Conclusion: </strong>These findings indicate that optimising the culture conditions of these two bacterial strains significantly enhances phosphatase production.