ERGA-BGE genome of the Spanish moon moth Actias isabellae Graells, 1849: a nocturnal lepidopteran protected by the Habitats Directive
High-quality reference genomes are critical resources for understanding biodiversity and supporting conservation efforts. We present the chromosome-level assembly of the Spanish moon moth, <i>Actias isabellae</i> (Graells, 1849), a nocturnal lepidopteran protected under the EU Habitats Directive. The assembly spans 0.56 Gb across 31 pseudomolecules, including the Z chromosome, with a contig N50 of 18.9 Mb and a scaffold N50 of 20.4 Mb. The mitochondrial genome was also assembled into a 15,247 bp circular sequence. Annotation identified 11,805 protein-coding genes and 2,238 non-coding genes, with a BUSCO completeness of over 94%. Notably, no W chromosome was detected, suggesting a ZZ/Z0 sex determination system. This reference genome provides an essential foundation for studying sex chromosome evolution in Lepidoptera and enables advanced population genomics monitoring of this protected species. More broadly, it contributes to ongoing efforts within the European Reference Genome Atlas and the Earth BioGenome Project to harness genomics for biodiversity conservation.
Authors
- Ana Riesgo (ORCID: https://orcid.org/0000-0002-7993-1523)
- Tyler Alioto (ORCID: https://orcid.org/0000-0002-2960-5420)
- Roger Vila (ORCID: https://orcid.org/0000-0002-2447-4388)
- Francisco Câmara Ferreira (ORCID: https://orcid.org/0000-0002-1971-5466)
- Thomas Marcussen (ORCID: https://orcid.org/0000-0002-1960-9508)
- Chiara Bortoluzzi (ORCID: https://orcid.org/0000-0001-6589-6635)
- Astrid Böhne (ORCID: https://orcid.org/0000-0002-1284-3115)
- Neus Marí‐Mena
- Rebekah A. Oomen (ORCID: https://orcid.org/0000-0002-2094-5592)
- Leanne S. Haggerty (ORCID: https://orcid.org/0000-0001-8843-3596)
- Carlos López‐Vaamonde (ORCID: https://orcid.org/0000-0003-2278-2368)
- Marta Vila (ORCID: https://orcid.org/0000-0002-8803-9174)
- Daniel García‐Souto (ORCID: https://orcid.org/0000-0002-0997-8799)
- Fernando Cruz (ORCID: https://orcid.org/0000-0003-4098-8829)
- Fergal J. Martin (ORCID: https://orcid.org/0000-0002-1672-050X)
- Laura Aguilera (ORCID: https://orcid.org/0000-0002-8631-8778)
- Jèssica Gómez‐Garrido (ORCID: https://orcid.org/0000-0001-6409-8009)
- Torsten H. Struck (ORCID: https://orcid.org/0000-0003-3280-6239)
- Rita Monteiro (ORCID: https://orcid.org/0000-0003-1374-4474)
- Marta Ortova Gut (ORCID: https://orcid.org/0000-0002-4063-7159)
Institutions
- University of New Brunswick (CA)
- Université de Tours (FR)
- Universidade da Coruña (ES)
- SIB Swiss Institute of Bioinformatics (CH)
- Centre National de la Recherche Scientifique (FR)
- European Bioinformatics Institute (GB)
- Natural History Museum (GB)
- Universitat Pompeu Fabra (ES)
- University of Oslo (NO)
- University of Agder (NO)
- Saint John Regional Hospital (CA)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Zoological Research Museum Alexander Koenig (DE)
- Museo Nacional de Ciencias Naturales (ES)
- Institut de Biologia Evolutiva (ES)
- Ecologie et Ecophysiologie Forestières (FR)
- Centro Nacional de Análisis Genómico (ES)
- University of Florence (IT)
- Universidade de Vigo (ES)
- Swiss Federal Institute of Aquatic Science and Technology (CH)
- Universitat de Barcelona (ES)
- University of Gothenburg (SE)
Publication Details
- Journal
- Open Research Europe
- Published
- 2025-09-16
- DOI
- https://doi.org/10.12688/openreseurope.20658.2
- Primary Topic
- Lepidoptera: Biology and Taxonomy
- Type
- article
- Field-Weighted Citation Impact
- 0.00