Methodological validation of Miro1 retention as a candidate Parkinson’s disease biomarker
Mitochondrial markers help stratify Parkinson's disease (PD) patients. We use high-throughput blotting to quantify Miro1, Mfn2, and VDAC levels in fibroblasts, blood cells, and iPSC-derived neurons. Miro1 is specifically retained in PD cells but degraded in healthy ones after mitochondrial depolarization. We correlate Miro1 retention scores with pathogenic mutations, genetic background, age, and clinical data. This scalable assay and quantifiable score for mitochondrial-PD support biomarker development and pharmacological screening.
Authors
- Kathrin Brockmann (ORCID: https://orcid.org/0000-0002-7515-8596)
- Daniel Merk (ORCID: https://orcid.org/0000-0003-2935-6919)
- Julia C. Fitzgerald (ORCID: https://orcid.org/0000-0002-3928-4878)
- Patrick May (ORCID: https://orcid.org/0000-0002-3954-0421)
- Christian Johannes Gloeckner (ORCID: https://orcid.org/0000-0001-6494-6944)
- Richard Wüst
- Giuseppe Arena (ORCID: https://orcid.org/0000-0003-2398-5503)
- Vyron Gorgogietas (ORCID: https://orcid.org/0000-0002-3211-6468)
- Thomas Gasser (ORCID: https://orcid.org/0000-0002-1069-1146)
- Daniele Ferrante (ORCID: https://orcid.org/0000-0002-2558-7149)
- Anne Grünewald (ORCID: https://orcid.org/0000-0002-4179-2994)
- Layla Drwesh (ORCID: https://orcid.org/0000-0002-1868-1487)
- Rejko Krüger (ORCID: https://orcid.org/0000-0003-4258-6241)
Institutions
- Centre Hospitalier de Luxembourg (LU)
- University of Luxembourg (LU)
- German Center for Neurodegenerative Diseases (DE)
- Luxembourg Institute of Health (LU)
- University Children's Hospital Tübingen (DE)
- Hertie Institute for Clinical Brain Research (DE)
- University of Tübingen (DE)
Publication Details
- Journal
- npj Parkinson s Disease
- Published
- 2025-09-15
- DOI
- https://doi.org/10.1038/s41531-025-01115-8
- Citations
- 1
- Primary Topic
- RNA regulation and disease
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Michael J. Fox Foundation for Parkinson's Research
- Eberhard Karls Universität Tübingen
- National Institute of Neurological Disorders and Stroke