Image analysis of sweet potato calluses in vitro

Abstract The objective of this work was to evaluate the use of the GroundEye equipment to characterize calluses from sweet potato explants. Sweet potato calluses from different explants (leaf, stem, and root) were grown in a culture medium with four growth regulators (naphtaleneacetic acid, 6-benzylaminopurine, 2,4-dichlorophenoxyacetic acid, and kinetin) and under two cultivation conditions (light and dark). The GroundEye system was used to analyze callus geometry and color, capturing color variation efficiently across different samples. In addition, the measurement of the area by the software directly reflects callus mass. Therefore, GroundEye is efficient in evaluating the geometry and color of calluses from explants of the Brazlândia Branca sweet potato cultivar.