Discovery and biosynthetic pathway speculation of aromatic polyketone rishirilide K

In our process of identifying novel anticancer and infection-resistant aromatic polyketones, four products (<b>1-4</b>) were isolated from <i>Streptomyces olivaceus</i> NA07600. Their structures were established based on extensive NMR data analyses. Meanwhile, the biosynthetic pathway of <i>Streptomyces olivaceus</i> NA7600 was proposed, with the rishirilide biosynthetic gene cluster (BGC) analysed and functionally annotated. Of four compounds (<b>1-4</b>), rishirilide K (<b>1</b>) is a new aromatic polyketone and the biosynthetic pathway differs from the reported biosynthetic Baeye-Villiger chemistry of compounds (<b>2-4</b>) in the side chain of the starting unit of biosynthesis.