Folate production in lactic acid bacteria and determination of folate derivatives by HPLC

zFolate is a B-group vitamin that humans cannot synthesize and must be taken externally.Although some types of Lactic Acid Bacteria can produce folate, there is insufficient information about the production of folate by yoghurt starter cultures.In this study, four bacteria belonging to two Lactobacillus, and two Streptococcus genera were isolated from yoghurt samples, and the isolates were biochemically identified by 16S rDNA with an API 50 CHL kit.Intracellular and extracellular folate production of the bacteria was designated in various media and buffer applications.The highest intracellular folate production was observed in Lactobacillus delbrueckii subsp.bulgaricus ZN951 (126.3 g/L) and Streptococcus.thermophilus Z151 (177.1 g/L) strains in MRS/Elliker medium with potassium phosphate buffer.L. delbrueckii subsp.bulgaricus ZN541 strain showed the highest extracellular folate production (105.1g/L) in the same medium and buffer, but S. thermophilus Z151 strain exhibited 86.4 g/L folate productions.Whether the strains contained folate derivatives and pABA was investigated by HPLC analysis and pABA was not detected in any of the strains.Among cultures, the highest total folate content was detected as 110.5g/L by L. delbrueckii subsp.bulgaricus ZN951 strain.This strain was found to have concentrations of 5-MeTHF and 5-FmTHF.Therefore, the use of these bacteria can be an alternative to naturally increase folate content in foods. Folat, insanlarn sentezleyemedikleri ve dardan alnmas gereken B grubu bir vitamindir. Baz Laktik AsitBakterileri folat retebilse de, yourt starter kltrlerinin folat retimi hakknda yeterli bilgi yoktur.Bu almada yourt rneklerinden iki Lactobacillus ve iki Streptococcus cinsine ait drt bakteri izole edilmi ve izolatlar API 50 CHL kiti ile 16S rDNA ile biyokimyasal olarak tanmlanmtr.Bakterilerin hcre ii ve hcre d folat retimi eitli ortam ve tampon uygulamalarnda belirlenmitir.En yksek hcre ii folat retimi Lactobacillus delbrueckii subsp.bulgaricus ZN951 (126.3 g/L) ve Streptococcus thermophilus Z151 (177.1 g/L) sularnda MRS/Elliker besiortam ve potasyum fosfat tamponunda belirlenmitir.L. delbrueckii subsp.bulgaricus ZN541 suu, ayn ortam ve tamponda en yksek hcre d folat retimini (105.1 g/L) gsterirken, S. thermophilus Z151 suu, 86.4 g/L folat retimi sergilemitir.Sularn folat trevleri ve pABA ierip iermedii HPLC analizi ile aratrlm ve sularn hibirinde pABA tespit edilememitir.Kltrler arasnda en yksek toplam folat ierii 110.5 g/L olarak L. delbrueckii subsp.bulgaricus ZN951 suunda tespit edilmitir.Bu suun 5-MeTHF ve5-FmTHF konsantrasyonlarna sahip olduu bulunmutur.