Pro-apoptotic activity of the antiviral and antitumor drug Izatizon

Aim. Apoptosis is a programmed cell death characteristic of all multicellular organisms, which is not accompanied by an inflammatory process, unlike necrosis, and is an immunologically inert process. To study the proapoptotic effect of isathizone and its analogues on the culture of the human malignant cell line K-562. Methods. Flow cytometry was used to detect the effects of drugs on apoptosis. The effect of drugs on caspase-3 activity was determined using Western blot. Results. Flow cytometry data show that isathizone and its analog Idd stopped cell division in the G0–G1 phase, after which apoptosis began. The data of studies using flow cytometry show that isatizone and its analogue Idd stopped cell division in the G0-G1 phase, after which apoptosis began. Studies on the effect of isathizone and its analogues on the activity of caspase-3 in the culture of malignant K-562 cells showed that all drugs initiate caspase-3. Conclusions. Izatizone and its analogues affect apoptosis of malignant K-562 cell cultures and can be used for further research in practical medicine.