Efficient Expression and Purification of Recombinant Mouse Dimeric IgA
Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.
Authors
- Hedda Wardemann (ORCID: https://orcid.org/0000-0003-3921-5933)
- K. L. Markova (ORCID: https://orcid.org/0000-0003-2748-3543)
- Andrew J. Macpherson (ORCID: https://orcid.org/0000-0002-7192-0184)
- Jens Schreiber (ORCID: https://orcid.org/0000-0001-6795-2282)
- Sophie Burkhalter
- Tim Rollenske
- Antonia Geisse
- Tao Zhang (ORCID: https://orcid.org/0000-0003-2765-2802)
Institutions
- University of Bern (CH)
- Bern University of Applied Sciences (CH)
- German Cancer Research Center (DE)
- Heidelberg University (DE)
- University Hospital Bonn (DE)
- University Hospital of Bern (CH)
Publication Details
- Journal
- European Journal of Immunology
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1002/eji.70055
- Citations
- 1
- Primary Topic
- T-cell and B-cell Immunology
- Type
- article
- Field-Weighted Citation Impact
- 0.80
Funders
- Deutsches Krebsforschungszentrum
- University of Bern