Plasmacytoid and CD141+ Myeloid Dendritic Cells Cooperation with CD8+ T Cells in Lymph Nodes is Associated with HIV Control
Dendritic cells (DC) are known to modulate antiviral immune responses; however, the knowledge about the role of different DC subsets in antiviral T cell priming in human tissues remains uncompleted. In the context of HIV infection, we determined the phenotype and location of plasmacytoid and CD141+ myeloid DCs (pDCs and mDCs) in lymph nodes of people living with HIV (PLWH). We found an interaction between pDCs and CD141+ mDCs with CD8+ T cells, being associated with participants' viral levels in blood and tissue. Moreover, we demonstrated a higher and more polyfunctional superantigen- and HIV-specific CD8+ T cell response after the coculture with Toll-like receptor (TLR)-primed pDCs and CD141+ mDCs. Last, we showed the potential of programmed cell death-1 (PD-1) blocking using pembrolizumab to further increase antigen-specific CD8+ T cell response along with TLR agonists. Therefore, these results showed a cooperation between pDCs, CD141+ mDCs and CD8+ T cells in lymph nodes of PLWH, which is associated with higher HIV control, highlighting the importance of DC subsets crosstalk to achieve a more potent anti-HIV response and support the use of DC-based immunotherapies for HIV control.
Authors
- Beatriz Domínguez-Molina
- Ezequiel Ruíz-Mateos (ORCID: https://orcid.org/0000-0001-6747-7813)
- Laura E. Via (ORCID: https://orcid.org/0000-0001-6074-9521)
- Joana Vitallé (ORCID: https://orcid.org/0000-0002-5292-1851)
- Sara Bachiller (ORCID: https://orcid.org/0000-0002-9000-3787)
- Alberto Pérez‐Gómez (ORCID: https://orcid.org/0000-0002-3644-2914)
- Sara Ferrando‐Martínez (ORCID: https://orcid.org/0000-0003-0721-555X)
- Richard A. Koup (ORCID: https://orcid.org/0000-0002-3090-7282)
- Carmen Gasca‐Capote (ORCID: https://orcid.org/0000-0002-7211-8599)
- Francisco José Ostos (ORCID: https://orcid.org/0000-0001-6583-6974)
- María Reyes Jiménez‐León (ORCID: https://orcid.org/0000-0003-2723-319X)
- Mohammed Rafii‐El‐Idrissi Benhnia (ORCID: https://orcid.org/0000-0001-7405-9476)
- Luís F. López‐Cortés (ORCID: https://orcid.org/0000-0002-3804-3458)
- Asunción Mochón (ORCID: https://orcid.org/0000-0002-2583-4875)
- María Inés Camacho-Sojo
- Eirini Moysi (ORCID: https://orcid.org/0000-0002-2070-2902)
- Constantinos Petrovas (ORCID: https://orcid.org/0000-0002-5067-5810)
- Isabel Gallego
Institutions
- National Institutes of Health (US)
- Instituto de Biomedicina de Sevilla (ES)
- National Institute of Research in Tuberculosis (IN)
- Hospital Universitario Virgen del Rocío (ES)
- Universidad de Sevilla (ES)
- University of Lausanne (CH)
Publication Details
- Journal
- MedComm
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1002/mco2.70354
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Health and Human Services
- Gilead Sciences
- Universidad de Sevilla
- European Commission
- Junta de Andalucía
- National Institutes of Health
- Consejo Superior de Investigaciones Científicas
- Instituto de Salud Carlos III
- Agencia Estatal de Investigación
- National Institute of Allergy and Infectious Diseases