New application of Pulsed Electric Fields technology to decellularize Ecklonia radiata as a sustainable cellulose matrix for tissue engineering

The development of tissue constructs relies heavily on scaffolding materials that support cell growth.These scaffolds must exhibit appropriate physical, mechanical, and biochemical properties essential for effective tissue repair.Decellularization techniques allow for the extraction of native extracellular matrix (ECM) from animal tissues, yielding biocompatible scaffolds for tissue engineering applications.Additionally, decellularized plant materials offer cellulose-based matrices that mimic ECM, presenting a sustainable, ethical, and cost-effective alternative.However, conventional decellularization processes predominantly use chemicals, raising concerns about their environmental impact.