Distinct roles of tandem acyl carrier protein domains in the assembly of a macrocyclic polylactone
Mutagenesis of the tandem acyl carrier protein (ACP<sub>1</sub>-ACP<sub>2</sub>) domains in the non-reducing polyketide synthase Men2 revealed their distinct roles in menisporopsin A biosynthesis. ACP<sub>1</sub> is essential for multiple esterifications and cyclolactonisation, whilst ACP<sub>2</sub> assembles the aromatic subunits of menisporopsin A. These findings provide a foundation for engineering macrocyclic polylactone compounds.
Authors
- Pakorn Wattana‐Amorn (ORCID: https://orcid.org/0000-0002-4780-7122)
- Thanakorn Deelee
Institutions
- Kasetsart University (TH)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc04497a
- Citations
- 1
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 1.39