AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice
We developed the AVID (ATP visualization in vivo directly) mouse, a genetically encoded biosensor mouse enabling real-time, multiscale imaging of ATP dynamics across the whole body, organs, and cellular compartments in living animals. AVID revealed previously undetectable localized ATP depletion near the central vein of the liver after myocardial infarction, spatially linked to kynurenic acid accumulation-a phenomenon invisible to conventional bulk metabolomics. By seamlessly integrating macroscopic organ-level imaging with microscopic spatial metabolomics, AVID establishes a new framework for spatial systems metabolism. Beyond myocardial infarction, this platform offers broad applicability to study organ-organ metabolic communication, spatial metabolic heterogeneity, and localized metabolic shifts across diverse physiological and pathological contexts, providing a transformative resource for metabolic research.
Authors
- Hiromi Imamura (ORCID: https://orcid.org/0000-0002-1896-0443)
- Kosuke Ino (ORCID: https://orcid.org/0000-0002-4750-4025)
- Takashi Izumi (ORCID: https://orcid.org/0000-0003-2898-3371)
- Masamichi Yamamoto (ORCID: https://orcid.org/0000-0002-9088-8044)
- Takahisa Anada (ORCID: https://orcid.org/0000-0001-8254-4199)
- Norimichi Koitabashi (ORCID: https://orcid.org/0000-0002-2113-3422)
- Makoto Suematsu (ORCID: https://orcid.org/0000-0002-7165-6336)
- Kaoru Hiramoto (ORCID: https://orcid.org/0000-0002-2210-7971)
- Naoki Matoba
- Daiki Setoyama (ORCID: https://orcid.org/0000-0002-4514-7546)
- Motoko Yanagita (ORCID: https://orcid.org/0000-0002-0339-9008)
- Shigenori Nonaka (ORCID: https://orcid.org/0000-0002-8093-0325)
- Takahisa Noma (ORCID: https://orcid.org/0000-0002-5964-0649)
- Yuki Sugiura (ORCID: https://orcid.org/0000-0002-6983-8958)
- Yuya Kunisaki (ORCID: https://orcid.org/0000-0003-0141-7038)
- Masahiko Kurabayashi (ORCID: https://orcid.org/0000-0002-9445-6759)
- Takahito Ayano (ORCID: https://orcid.org/0000-0002-1831-939X)
- Hinako Matsuda (ORCID: https://orcid.org/0000-0002-5271-6583)
- Riki Ogasawara (ORCID: https://orcid.org/0000-0002-2600-1613)
- Hideki Miwa (ORCID: https://orcid.org/0000-0001-6015-3007)
- Ryuto Yasui
- Yu‐ichiro Ohnishi (ORCID: https://orcid.org/0000-0003-3030-6230)
- Seri Kitada
Institutions
- Kyushu University (JP)
- National Institute for Basic Biology (JP)
- Gunma University (JP)
- Yamaguchi University (JP)
- Nagoya Institute of Technology (JP)
- Tohoku University (JP)
- Central Institute for Experimental Animals (JP)
- Kyoto University (JP)
- Kyushu University Hospital (JP)
- National Center of Neurology and Psychiatry (JP)
- National Cerebral and Cardiovascular Center (JP)
Publication Details
- Journal
- Cell Reports
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1016/j.celrep.2025.116246
- Citations
- 3
- Primary Topic
- Cancer, Hypoxia, and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 2.81