Non-standardized protein background in IVF media linked to serum-derived albumin supplementation
Serum-derived HSA was identified as the primary source of protein contamination in IVF media. This overlooked protein background contributes to variability in clinical culture conditions, undermines the reproducibility of secretome analyses, and complicates the discovery of reliable biomarkers in SCM.
Authors
- Drahomíra Kyjovská
- Zuzana Holubcová (ORCID: https://orcid.org/0000-0002-4658-6161)
- Volodymyr Porokh (ORCID: https://orcid.org/0000-0002-3586-8075)
- Pavel Otevřel
- Soňa Kloudová
- Markéta Nezvedová (ORCID: https://orcid.org/0000-0001-8130-1603)
- Tami Bočková
- Václav Pustka (ORCID: https://orcid.org/0009-0005-9857-3601)
- Barbora Maierová
Institutions
- Central European Institute of Technology (CZ)
- Masaryk University (CZ)
- University Hospital Brno (CZ)
Publication Details
- Journal
- Journal of Assisted Reproduction and Genetics
- Published
- 2025-09-13
- DOI
- https://doi.org/10.1007/s10815-025-03616-0
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 0.00