Protoplasting of Aspergillus niger using Trichoderma harzianum culture medium after growth on cell walls of the target fungus

Protoplast yield from Aspergillus niger can be low when using commercial lytic enzymes from Trichoderma harzianum. This study shows that the culture medium of T. harzianum after growth on A. niger cell walls is much more effective in protoplasting than commercial lysing enzymes.