Comparative molecular detection and phylogenetic analysis of Babesia canis vogeli in naturally infected dogs using two 18S rRNA primer sets in Khon Kaen, Thailand

<i>B. canis vogeli</i> was the only subspecies identified in naturally infected dogs in Khon Kaen during the study period. Babf/Babc demonstrated superior diagnostic sensitivity for <i>B. canis vogeli</i>, whereas Bab7/Bab9 offered broader detection, including <i>H. canis</i>. Phylogenetic analysis revealed close genetic relationships with isolates worldwide. These findings support the use of Babf/Babc for specific diagnosis and Bab7/Bab9 for broader screening in endemic regions.