Characterization of Antimicrobial Compounds from Abroma Augusta Leaf Extract

Background: Abroma augusta is used extensively in traditional medicine, but little is known about its antimicrobial components. Objective:The objective is to isolate, characterize and assess antimicrobial components from chloroform leaf extracts of A. Agusta. Methods:The leaves were extracted using chloroform and then fractionated using column chromatography based on activity.IR, H-NMR, and C-NMR spectroscopy were used to provide structural information about the isolated molecules.Antimicrobial activity was evaluated using disc diffusion and MIC tests against pathogenic fungus and both Gram-positive and Gram-negative bacteria.Results: Octacosanol and lupeol were the two chemicals that were purified.MIC values ranging from 32 to 128 g/ml, depending on the test organisms, indicated that both exhibited antibacterial activity.Antifungal activity was also observed against Candida albicans, Aspergillus niger, and Fusarium vasinfectum. Conclusion:A. augusta's ethnomedicinal use and promise as a source of natural antimicrobial agents are supported by the antimicrobial qualities of octacosanol and lupeol.