Protocol to perform assay for chromatin-bound condensates by exploratory sequencing in mouse embryonic stem cells

Chromatin-related protein condensates are integral to nuclear functions including transcriptional regulation and chromatin structure. Here, we present a protocol for detecting the occupancy of chromatin-associated condensates using the assay for chromatin-bound condensates by exploratory sequencing (ACC-seq) in mouse embryonic stem cells (mESCs). The workflow encompasses cell preparation, condensate modulation, library construction, and comprehensive sequencing analysis, enabling unbiased, genome-wide mapping of potential condensate occupancy across diverse chromatin states. For complete details on the use and execution of this protocol, please refer to He et al.¹.