DNA self-assembly encoding in spatial multi-omics imaging

<h2>Summary</h2> With the development of omics technology, multiple omics techniques have greatly expanded the effective data volume and shown great value in accurately diagnosing major diseases. However, the extremely limited clinical samples and a wide variety of detection biomarkers raise the key technical issue of how to use a small number of samples to achieve multiple biomarker detection. DNA self-assembly (DSA) encoding technology is a controllable technique that can achieve precise regulation of detection signals by combining fluorescent molecular markers based on the high programmability of DNA and the high predictability of the assembled products, playing an important role in the field of target multiplex analysis. Here, we provide a comprehensive overview of DSA encoding in spatial multi-omics imaging, further point out current problems in multiplex analysis, and put forward future research directions.