Fenugreek [Trigonella graecum] Methanolic Seeds Extract for Antioxidant (Superoxide and Singlet Oxygen Radical Scavenging), Antifungal Effect and Screening of Its Phytochemical Constitutes Using GC-MS

One of the plants that have antioxidant properties is known as Fenugreek with a scientific name of Trigonella foenum-graecum. This can be attributed to polyphenol content of the herb which consists of: flavonoids, phenolic acids, and tannins. Culturally fenugreek is used in traditional medicine because it has antioxidant properties that are applied in the treatment of blood sugar and cholesterol levels. A small market was taken to get the fresh seeds of Trigonella (foenum grecum). A diluted alcohol was applied to remove the sediment or traces of water as 10 grams of powdered seed was soaked in 80 percent alcohol. The so obtained filtrate was filtered through Whatmann filter paper No. 41 taking 2 grams of sodium sulphate. Phthalate -D 4, Methyl D-glucopyranoside; M ethyl, 2-Furanmethanol, a-ethyl, 3-O-Methyl-d -glucose, Adenine arabinoside, 1-Aziridineethanamine, 4-Tridecyne, palmitic acid, Pentanal, 2-methyl, gamma -Tocopherol, Piperid In singlet oxygen scavenging, the antioxidant activity was the following; crude (methanolic extract)- 53.18±4.39, ethyl acetate- 53.18±4.39, ethanol- 53.18±4.39, hexane fraction- 53.18±4.39, water fraction- 53.18±4.39, and Lipoic acid (standard)- 53.18±4.39. In superoxide radical scavenging, the antioxidant activity was 19.60±1.01, 23.76±1.30, 26.36±1.39, 21.46±1.07, 29.31±1.42, and 39.82±2.07. The antifungal activity of methanolic crude extract and ethanol fraction was compared to the standard and found to be bioactive natural component. The concentrations of itraconazole and flucytosine in the Fenugreek seeds extract (Trigonella graecum) against Penicillium digitatum were; 22.78±0.32, 16.88±0.26, 32.08±0.58 and 26.00±0.53 respectively and against Penicillium italicum 12.27±0.20, 13.97±0.21, 30.05 Against the Tricophyton rubrum, the antifungal activity came to be 11.05±0.17, 17.63±0.26, 23.13±0.28 and 28.89±0.41. Recorded 10.00l±0.14, 08.65l±0.10, 34.00l±0.59 and 29.16l±0.54 respectively against Microsporum Canis.