Quantitative Determination of α-Tocopherol and α-Tocopheryl Acetate in Pharmaceutical and Supplementary Soft Capsules by High Performance Liquid Chromatography

Aim: This study aims to determine the amounts of α-tocopherol (α-toc) and α-tocopheryl acetate (α-tocAc) in soft pharmaceutical capsules and supplements using a chromatographic method. Additionally, method validation parameters for both analytes were evaluated. Method: Analyses were performed using a chromatographic method. As part of the method validation, recovery (% recovery), repeatability (% RSD), linear regression coefficient (R²), limit of detection (LOD), and limit of quantification (LOQ) values were determined. Results: The recovery values were found to be above 95%. Repeatability (%RSD) was calculated as <3%. The linearity (R²) values were obtained as 0.999. For α-toc: LOD: 0.131 µg/mL and 2.228 µg/mL, LOQ: 0.396 µg/mL and 6.752 µg/mL. For α-tocAc: LOD: 0.260 µg/mL, LOQ: 0.787 µg/mL. Conclusion: The chromatographic method used in this study enabled the determination of α-toc and α-tocAc with high accuracy and repeatability. This method can be considered a reliable alternative for the analysis of these compounds in pharmaceutical products.