Quantitative analysis of gallic acid present in triphala in a polyherbal formulation

Triphala is well known Ayurvedic formulation in official Ayurvedic Formulary of India. It is used for immune system stimulation, improvement of digestion, relief of constipation, gastrointestinal tract cleansing, relief of gas, treatment of diabetes, eye disease, anaemia, jaundice, asthma, fever, chronic ulcers etc. Standardization of herbal formulation is essential in order to assess the quality, purity, safety and efficacy of the drug. In this study an attempt to evaluate the quantification of Gallic acid by High Performance Liquid Chromatography (HPLC) instrument. The percentage of free Gallic acid is prominent in our formulation compared to the standard was found to be under the specified limits.