Unraveling Seasonal Dynamics of Dissolved Organic Matter in Agricultural Ditches Using UV-Vis Absorption and Excitation–Emission Matrix (EEM) Fluorescence Spectroscopy

Seasonal dynamics of dissolved organic matter (DOM) in agricultural ditches significantly impact carbon cycling and water quality in connected rivers. This study aimed to characterize seasonal variations in DOM composition and dynamics within hierarchical agricultural ditch systems in Tianjin, northern China. Surface water samples were collected from river channels, main ditches, branch ditches, lateral ditches, and field ditches during wet (June 2021) and dry (December 2021) seasons. DOM characteristics were analyzed using dissolved organic carbon (DOC) quantification, ultraviolet-visible (UV-Vis) absorption spectroscopy, and three-dimensional excitation–emission matrix spectroscopy (3D-EEMs) coupled with parallel factor analysis (PARAFAC). The concentration of DOC in ditch surface water exhibited significant seasonal variations, with significantly higher levels observed during the wet season (Huangzhuang: 6.72 ± 0.7 mg/L; Weixing: 13.15 ± 3.1 mg/L) compared to the dry season (Huangzhuang: 5.93 ± 0.3 mg/L; Weixing: 9.35 ± 2.6 mg/L). Both UV-Vis spectral and EEM-PARAFAC analysis revealed that DOM in ditch systems was predominantly composed of fulvic-like and tryptophan-like components, representing the portion of organic matter in water bodies that is highly biologically active, highly mobile, relatively “fresh”, or “not fully humified”. PARAFAC identified microbial humic-like (C1: wet season 40.36%, dry season 34.42%) and protein-like (C3: wet season 40.3%, dry season 49.87%) components as dominant. DOM sources were influenced by dual inputs from terrestrial and autochthonous origins during the wet season, while primarily deriving from autochthonous sources in the dry season. This study elucidates the advances of spectroscopic techniques in deciphering the composition, sources, and influencing factors of DOM in aquatic systems. The findings support implementing riparian buffer strips and optimized fertilizer management to mitigate seasonal peaks of bioavailable DOM in agricultural ditch systems.