Tetrasaccharide Resin Glycosides with Nitric Oxide Production Inhibitory Activity from <i>Cuscuta campestris</i>
LC-HRMS/MS-based molecular-network-guided chemical investigation of <i>Cuscuta campestris</i> led to the isolation of seven undescribed tetrasaccharide-type resin glycosides (<b>1</b>-<b>7</b>). Their structures were elucidated using 1D and 2D NMR and HRESIMS analysis. Isolated resin glycosides were comprised of d-glucose, d-fucose, d-quinovose, and l-rhamnose, and these monosaccharides were partially acylated with acetyl, isobutyryl, <i>n</i>-hexanoyl, and niloyl organic acids. The absolute configuration of aglycones were determined as <i>S</i>-configuration using Mosher's method after acid hydrolysis of resin glycoside fraction. All compounds were evaluated for their inhibitory activity against the production of nitric oxide in RAW 264.7 cells. Compounds <b>2</b> and <b>3</b> exhibited inhibitory activity, with IC<sub>50</sub> values of 14.3 and 10.4 μM, respectively.
Authors
- Jae Sang Han
- Bang Yeon Hwang (ORCID: https://orcid.org/0000-0002-4148-6751)
- Jin‐Young Jang (ORCID: https://orcid.org/0000-0003-3312-0503)
- Jin Woo Lee
- Dongho Lee (ORCID: https://orcid.org/0000-0003-4379-814X)
- Mi Kyeong Lee (ORCID: https://orcid.org/0000-0001-5814-2720)
- Yong Beom Cho (ORCID: https://orcid.org/0000-0001-6486-2345)
- Jun Gu Kim
- Vu Quan Dang
Institutions
- Chungbuk National University (KR)
- Korea University (KR)
- Department of Biotechnology (IN)
- Institute of Plant Biology and Biotechnology (KZ)
- Drug Discovery Laboratory (Norway) (NO)
- Duksung Women's University (KR)
Publication Details
- Journal
- Journal of Natural Products
- Published
- 2025-09-09
- DOI
- https://doi.org/10.1021/acs.jnatprod.5c00713
- Primary Topic
- Natural product bioactivities and synthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00