Neural Plate Cell Manipulation Through In Utero Nanoinjection in an Embryonic Mouse Model

Source: Mangold, K., H el. al., Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5. J. Vis. Exp. (2022)This video demonstrates the in-utero nano-injection method for neural plate targeting in an embryonic mouse model. A high-titer lentiviral solution is injected into the amniotic cavity, where it integrates into neural plate cells, enabling stable gene manipulation for neurodevelopmental research and therapeutic applications.