Gene Expression via Single-Cell Electroporation in Mouse Hippocampal Slice Cultures

Source: Keener, D. G., et al. Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons. J. Vis. Exp. (2020).This video demonstrates the process of single-cell electroporation to introduce plasmid DNA into neurons within mouse hippocampal slice cultures. The procedure involves precise pipette positioning, pressure adjustments, and electroporation pulses to transiently permeabilize the neuronal membrane, facilitating gene transfer. After electroporation, the slice is incubated to enable the expression of the introduced gene of interest.