Poster Session A

performed with aortic VSMC isolated from mice carrying two floxed alleles of the Lpp gene.AAV9-mediated expression of Cre recombinase resulted in complete inactivation of the Lpp gene in these cells within 24 hours.Acute loss of Lpp produced similar effects in the VSMC than chronic Lpp deficiency with cells exhibiting increased proliferation and migration but decreased contractile and relaxant capacity.Bulk RNA-sequencing analysis of Lpp-KO VSMCs exposed to cyclic stretch demonstrated a prominent pro-inflammatory gene expression profile as well as changes in the expression of genes encoding proteins involved in extracellular matrix homeostasis. ConclusionsIn a nutshell, LPP plays a fundamental role in VSMC phenotype control and may protect the vessel wall from hypertension-induced arterial remodelling.