The Effect Formulation on The Activity of Catechins as A Sunscreen and Skin Lightening

Catechins are found in many plants, one of which comes from gambier. The stability of catechins which is affected by pH and temperature also affects the activity test after being made in dosage form. This study aims to see the effect of catechin formulations in the form of gel and nanoemulsion on the activity of sunscreen and skin lightening. Tests carried out in vitro included antioxidant activity tests using the DPPH method and B16F0 cell viability against catechins, determination of Sun Protecting Factor (SPF), Protection grade UVA (PA), tyrosinase and melanogenesis inhibition using the spectrophotometric method of catechin formulas in the form of gel and nanoemulsion dosage forms. The results showed that catechins have very strong antioxidant activity with an IC50 of 16.421 µg/mL, not toxic to B16F0 cells up to a concentration of 200 µg/mL. Catechins gel and nanoemulsion dosage forms have good stability during storage. The formulation had a significant effect (p<0.05) on the value of SPF, PA, percentage of tyrosinase, and melanogenesis inhibition. Catechins in the form of nanoemulsion formulation provide better sunscreen and skin lightening activity than those in gel dosage forms.