CD33 <sup>KO</sup> -CD33-mesothelin Loop CAR design avoids fratricide and improves efficacy of iNK cells against acute myeloid leukemia
Loop CAR empowered both UCB-NK cells and hPSC-iNK cells with superior cytotoxicity against CD33<sup>+</sup>MSLN<sup>+</sup> tumor cells. Genetic disruption of CD33 avoided fratricide and improved efficacy of Loop CAR-iNK cells against AML. This innovative strategy possesses unique advantages and translational potential for treating AML.
Authors
- Qitong Weng (ORCID: https://orcid.org/0009-0003-3929-4384)
- Jiacheng Xu (ORCID: https://orcid.org/0000-0003-4753-0634)
- Qi Zhang (ORCID: https://orcid.org/0000-0001-8939-4111)
- Quan Chen (ORCID: https://orcid.org/0000-0001-7539-8728)
- Xiujuan Zheng (ORCID: https://orcid.org/0000-0002-4703-9530)
- Yanhong Liu (ORCID: https://orcid.org/0000-0001-6999-7062)
- Dehao Huang (ORCID: https://orcid.org/0009-0006-2693-9309)
- Xin Du (ORCID: https://orcid.org/0000-0002-9004-8758)
- Mengyun Zhang (ORCID: https://orcid.org/0000-0003-1505-3757)
- Leqiang Zhang (ORCID: https://orcid.org/0000-0003-1439-1287)
- Tianyuan Hu (ORCID: https://orcid.org/0000-0002-8145-5802)
- Ziyun Xiao
- Minming Li (ORCID: https://orcid.org/0000-0002-7370-6237)
- Chengxiang Xia (ORCID: https://orcid.org/0000-0002-7923-2802)
- Yunqing Lin (ORCID: https://orcid.org/0000-0002-6743-954X)
- Lijuan Liu (ORCID: https://orcid.org/0000-0002-8326-0371)
- Fan Zhang (ORCID: https://orcid.org/0000-0003-2182-7018)
- Yi Chen (ORCID: https://orcid.org/0000-0002-4891-8289)
- Pengcheng Liu (ORCID: https://orcid.org/0000-0002-1860-5118)
- Zhiqian Wang (ORCID: https://orcid.org/0000-0001-9831-649X)
- Jinyong Wang (ORCID: https://orcid.org/0000-0002-7218-0659)
- Fangxiao Hu (ORCID: https://orcid.org/0009-0000-9376-6870)
- Xiaofan Zhu (ORCID: https://orcid.org/0000-0001-7987-7793)
- Wenbin Qian (ORCID: https://orcid.org/0000-0002-9817-6674)
- Yanping Zhu (ORCID: https://orcid.org/0000-0003-4524-0350)
- Chenyuan Zhang (ORCID: https://orcid.org/0000-0002-2706-4237)
- Tongjie Wang (ORCID: https://orcid.org/0000-0003-1207-8377)
- Jiaxin Wu
- Hanmeng Qi
- Yiyuan Shen (ORCID: https://orcid.org/0009-0000-5954-270X)
- Yao Wang (ORCID: https://orcid.org/0009-0003-6905-0612)
- Yaoqin Zhao
- Aibin Liang
- Wenyu Yang (ORCID: https://orcid.org/0009-0009-1946-0940)
Institutions
- Tongji University (CN)
- Chinese Academy of Sciences (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Guangzhou Institutes of Biomedicine and Health (CN)
- Guangdong Academy of Medical Sciences (CN)
- Institute of Hematology & Blood Diseases Hospital (CN)
- Guangdong Provincial People's Hospital (CN)
- Second Affiliated Hospital of Zhejiang University (CN)
- Tongji Hospital (CN)
- University of Chinese Academy of Sciences (CN)
- Institute of Zoology (CN)
Publication Details
- Journal
- Journal for ImmunoTherapy of Cancer
- Published
- 2025-09-01
- DOI
- https://doi.org/10.1136/jitc-2025-011887
- Citations
- 2
- Primary Topic
- CAR-T cell therapy research
- Type
- article
- Field-Weighted Citation Impact
- 2.09
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- National Science and Technology Major Project
- Institute of Zoology, Chinese Academy of Sciences
- National Key Research and Development Program of China