Optogenetic Manipulation of Neural Circuits for the Sleep-to-Wakefulness Transition in Mice

Source: Kodani, S., et.al. Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice. J. Vis. Exp. (2019)This video demonstrates the optogenetic manipulation of neural circuits involved in the sleep-to-wakefulness transition in mice. The procedure involves surgically implanting an optic fiber and electrodes into the brain and muscles to enable light stimulation and electrophysiological recording. By delivering light to activate light-sensitive cation channels in the bed nucleus of the stria terminalis (BNST), neuronal activity is modulated, allowing researchers to analyze brain and muscle signals associated with wakefulness induction.