Stereotaxic Injection of Viruses for Stable Genetic Modification in a Mouse Model

Source: Porlan, E., et al. Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects. J. Vis. Exp. (2016)This video demonstrates stable genetic modification of neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ) through stereotaxic injection of a virus-carrying therapeutic gene in a mouse model. The virus enters NSCs, undergoes reverse transcription, integrates into the genome, and enables stable and efficient genetic modification for neurotherapeutic applications.