Gene Transfer into Schwann Cells of the Rodent Sciatic Nerve via Electroporation

Source: Ino, D. et. al., In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation. J. Vis. Exp. (2016)This video demonstrates in vivo gene transfer to Schwann cells of the rodent sciatic nerve using electroporation. A plasmid DNA–dye solution is injected into the nerve, and electric pulses facilitate DNA entry. The DNA later integrates into the cell’s genome, enabling successful gene transfer in Schwann cells.