Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation

Source: Silva, C. et al. Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections. J. Vis. Exp. (2020)This video demonstrates the protocol for microinjection of tetrodotoxin (TTX) into the hypothalamus of a freely moving rat to transiently inhibit neuronal activity, suppress gonadotropin-releasing hormone secretion, and prevent ovulation.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-08-29
DOI
https://doi.org/10.3791/23841
Primary Topic
Marine Toxins and Detection Methods
Type
article
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article

Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation

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Journal of Visualized Experiments
Marine Toxins and Detection Methods
article

Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation

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Abstract

Source: Silva, C. et al. Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections. J. Vis. Exp. (2020)This video demonstrates the protocol for microinjection of tetrodotoxin (TTX) into the hypothalamus of a freely moving rat to transiently inhibit neuronal activity, suppress gonadotropin-releasing hormone secretion, and prevent ovulation.

Journal of Visualized Experiments
Openalex Percentile: Top 80%
Marine Toxins and Detection Methods
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