Transplantation of Stem Cell-Derived Neuronal Progenitors into Human Cortical Slices

Source: Palma-Tortosa, S., et.al. Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation. J. Vis. Exp. (2022)This video demonstrates the transplantation of stem cell-derived neuronal progenitor cells into human cortical tissue slices. The procedure involves isolating and resuspending progenitor cells in a basement membrane matrix, followed by their injection into a cortical slice placed on a transwell assembly. After incubation to allow matrix solidification and neuronal differentiation, the transplanted tissue is prepared for further analysis.