THE PUTATIVE REGENERATIVE CAPABILITY OF CELASTROL AND ITS NANOEMULSION ON OSTEOPOROTIC RATS EXPERIMENTALLY INDUCED BY OVARIECTOMY

Introduction: Postmenopausal osteoporosis (PMOP) is the most common bonedisease in the developed world with serious public health concerns. Celastrol (CEL) hasbeen voted as one of the top five promising natural medicine molecules which may helpin PMOP management. Celastrol nanoemulsion (CTL-NE) was proved to improve theoral bioavailability, therapeutic effects of CTL. Aim of the study: To detect and comparethe effect of CTL and CTL-NE on mandibular bone architecture and bone turnovermarkers (BTMs) in ovariectomized (OVX) rats as a model of PMOP. Materials andMethods: Two groups of sixty female rats; the OVX group and the sham group, wereassigned at random. The rats in each group were further separated into three equalsubgroups sixty days after OVX and sham operations. The rats in subgroup 1 werenot given any treatment, those in subgroup 2 were given CTL, and those in subgroup3 were given CTL-NE for four weeks. At the end of the experiment, blood sampleswere collected for biochemical analysis of bone turnover markers (BTMs), then therats were euthanized, and their lower jaws were prepared for light microscope (LM).Results: Histological examination of OVX rats mandibular bone revealed compromisedbone architecture and significant increase in BTMs compared with the normal shamones. Interestingly OVX group showed notable improvement in bone microstructureand marked decrease in BTMs upon celastrol administration, however, the superiorimprovement was achieved in CTL-NE treated rats and these results were confirmedhistologically and biochemically. Conclusion: Systemic administration of CTL andCTL-NE may be effective therapy for treatment of poor bone quality in OVX inducedosteoporosis, with privilege to CTL-NE therapy.