Isolation and characterization of a bioactive compound with pharmacological potential from Salacia oblonga endophytome

The current work emphasizes the isolation and characterization of the bioactive compound from an endophytic fungus isolated from medicinal plant Salacia oblonga. Antimicrobial, antioxidant and antidiabetic activities of the active fraction were confirmed using standard methods. The extracts showed significant antibacterial activity against some pathogenic bacteria and were also positive to in vitro antidiabetic assays. The IC50 values obtained in the antioxidant assays (34.56 μg for SOEF fraction and 19.65 μg for ascorbic acid in the case of DPPH assay and 38.34 μg for SOEF fraction and 19.65 μg for quercetin in the case of ABTS assay) were positive and comparable to the standards used. The purified active fraction was identified and characterized using LC-MS, NMR, FTIR, Homonuclear correlation spectroscopy sequence (COSY) and Heteronuclear Multiple Bond Coherence (HMBC). From the studies, the compound obtained in the active fraction was found to be 3-(4-hydroxyphenyl) propionic acid, a potent antioxidant compound that has several applications in food and pharma industries.