Egg Yolk Granule Nanoparticles Promote Longitudinal Bone Growth in HFD-Obese Mice

The relationship between obesity and bone development remains uncertain and requires further study. Egg yolk granules (EYGs), due to their high content of phosvitin (PV), are speculated to have the potential to promote bone growth. And EYGs nanoparticles (EYG NPs) may help improve their digestibility. This study aimed to evaluate the effects of EYG NPs on longitudinal bone growth in high-fat diet-induced obese mice. EYG NPs were prepared by treating EYGs with (NaPO₃)₆ and ultrasonication, then characterized. The simulated gastrointestinal digestion experiment indicated that the modification of EYG significantly enhanced the digestibility of PV. After 12 weeks of intervention, body growth indicators, serum bone metabolism markers, tibial length, bone mineral density (BMD) and growth plate height were measured. In obesity model, the body length increased, while serum ALP activity decreased, and BMD showed no differences compared to those in Normal group. High-dose EYG NPs supplementation promoted longitudinal bone growth of obese mice, as evidenced by increased tibial length, elevated serum ALP activity, and enhanced growth plate height, while maintaining BMD. These findings suggest that EYG NPs have the potential of promoting longitudinal bone growth among the adolescent obese population.