Comparative Evaluation of Three Primary Antibody Clones for p16 Immunohistochemistry in Gynecologic Tumors
Our findings indicate that E6H4, JC8, and 6H12 clones yield comparable staining results when used in conjunction with standardized automated protocols. These results support the practical interchangeability of these clones in clinical and research settings, particularly when cost, availability, or risk management require substitution. Laboratories should continue to perform internal validation and utilize external quality assurance programs when implementing p16 IHC.
Authors
- Ayumi Sugitani
- Mayumi Kobayashi Kato (ORCID: https://orcid.org/0000-0003-4065-0289)
- Hiroshi Yoshida (ORCID: https://orcid.org/0000-0002-7569-7813)
- Masaya Uno (ORCID: https://orcid.org/0000-0001-8549-1635)
- Mitsuya Ishikawa (ORCID: https://orcid.org/0000-0001-8855-2966)
Institutions
- National Cancer Center Hospital East (JP)
Publication Details
- Journal
- Antibodies
- Published
- 2025-09-05
- DOI
- https://doi.org/10.3390/antib14030077
- Primary Topic
- Cervical Cancer and HPV Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00