Genetic Polymorphisms of Invasive Ambrosia artemisiifolia L. in Localities of Slovakia Accessed by Bet v 1 Homologs Differ in Discrimination of Accessions and Show Their Outcrossing in This Area

<i>Ambrosia artemisiifolia</i> is one of the invasive plants found in Europe. Research into this species is important not only in terms of environmental consequences but also in terms of human health, as it is an allergenic weed. Here, the genetic variability in the accessions of common ragweed from three localities of the Slovak Republic was analysed. A Bet v 1-based amplified polymorphism was applied for degenerated and non-degenerated primers. Three of the five primer variants were able to distinguish all analysed accessions, and two primer combinations were able to distinguish two accessions in the same amplified fingerprint profile from Kicsina. Using the BBAP technique, between of 1 to 16 amplicons were obtained per 1 genotype of common ragweed within a range of 68 to 3266 base pairs. Using two of the BBAP primer variants, unique fragments were obtained. The results indicate that the outcrossing among Slovak genotypes of <i>Ambrosia artemisiifolia</i> L. as non-specific BBAP profiles of individual analysed localities was observed.