Advancing Preclinical Biology for Ewing Sarcoma: An International Effort
Ewing sarcoma is an aggressive bone and soft-tissue cancer affecting adolescents and young adults. In vitro and in vivo models of Ewing sarcoma have been instrumental in advancing our understanding of Ewing sarcoma biology and essential in evaluating potential therapies, particularly for metastatic or relapsed disease for which effective treatment options remain limited. Through an international collaborative effort between the Children's Oncology Group Bone Tumor Committee and the Euro Ewing Consortium, we review the current landscape of preclinical modeling used in Ewing sarcoma research encompassing both in vitro (cell lines and tumor organoids) and in vivo (mouse and nonmammalian xenografts) model systems. We discuss factors that can influence experimental results, provide testing considerations for both in vitro and in vivo studies, and descriptions of existing preclinical data repositories. We highlight current needs in Ewing sarcoma modeling and the importance of enhanced international cooperative research and patient advocacy efforts which will be critical in expanding our resources of biologically relevant Ewing sarcoma models to enable translation of preclinical findings into effective therapeutic strategies for patients with Ewing sarcoma.
Authors
- Tamar Y. Feinberg (ORCID: https://orcid.org/0000-0003-2897-036X)
- Shireen Ganapathi (ORCID: https://orcid.org/0000-0002-1239-2166)
- Brian D. Crompton (ORCID: https://orcid.org/0000-0001-9404-6621)
- Thomas G. P. Grünewald (ORCID: https://orcid.org/0000-0003-0920-7377)
- Katia Scotlandi (ORCID: https://orcid.org/0000-0001-6114-9499)
- Christine M. Heske (ORCID: https://orcid.org/0000-0003-0956-6249)
- Elizabeth R. Lawlor (ORCID: https://orcid.org/0000-0002-2350-1540)
- Sarah Cohen‐Gogo (ORCID: https://orcid.org/0000-0002-8852-1104)
- Alice Soragni (ORCID: https://orcid.org/0000-0002-6634-2885)
- Heinrich Kovar (ORCID: https://orcid.org/0000-0001-6873-9109)
- Patrick J. Grohar (ORCID: https://orcid.org/0000-0002-1071-4563)
- Olivier Delattre (ORCID: https://orcid.org/0000-0002-8730-2276)
- Damon R. Reed (ORCID: https://orcid.org/0000-0002-8238-2465)
- David M. Loeb (ORCID: https://orcid.org/0000-0002-7319-5000)
- Susan A. Burchill (ORCID: https://orcid.org/0000-0002-1034-3723)
- Joshua O. Nash (ORCID: https://orcid.org/0009-0003-2718-2262)
- Joyce E. Ohm (ORCID: https://orcid.org/0000-0002-7390-8701)
- Dusan Pesic (ORCID: https://orcid.org/0009-0007-4201-0257)
- Adam Shlien (ORCID: https://orcid.org/0000-0002-0368-5370)
- Filemon S. Dela Cruz (ORCID: https://orcid.org/0000-0002-8356-5191)
- Jessica D. Daley (ORCID: https://orcid.org/0000-0001-5133-448X)
- Eleni M. Tomazou (ORCID: https://orcid.org/0000-0002-7497-4567)
- Jaume Mora (ORCID: https://orcid.org/0000-0002-9386-5980)
- Kelly M. Bailey (ORCID: https://orcid.org/0000-0003-3925-3243)
- Elizabeth A. Roundhill (ORCID: https://orcid.org/0000-0002-0508-3648)
- James F. Amatruda (ORCID: https://orcid.org/0000-0002-9901-2137)
- Didier Surdez (ORCID: https://orcid.org/0000-0002-7118-7859)
- Elizabeth Stewart (ORCID: https://orcid.org/0000-0001-8005-0427)
- Jaime Alvarez-Perez
Institutions
- University of Southern California (US)
- Roswell Park Comprehensive Cancer Center (US)
- Seattle Children's Hospital (US)
- Albert Einstein College of Medicine (US)
- Hospital Sant Joan de Déu Barcelona (ES)
- University of Leeds (GB)
- St. Jude Children's Research Hospital (US)
- Memorial Sloan Kettering Cancer Center (US)
- Children's Hospital of Los Angeles (US)
- Inserm (FR)
- University of California, Los Angeles (US)
- University of Pittsburgh (US)
- German Cancer Research Center (DE)
- University of Zurich (CH)
- Heidelberg University (DE)
- Université Paris Sciences et Lettres (FR)
- University of Michigan (US)
- Hospital for Sick Children (CA)
- St James's University Hospital (GB)
- University Hospital Heidelberg (DE)
- Comprehensive Cancer Center Vienna (AT)
- University Hospital of Zurich (CH)
- Seattle Children's Hospital (US)
- Center for Cancer and Blood Disorders (US)
- National Center for Tumor Diseases (DE)
- Michigan Medicine (US)
- Hopp Children's Cancer Center Heidelberg (DE)
- Dana-Farber Cancer Institute (US)
- Cancer Research Institute of the Slovak Academy of Sciences (SK)
- Istituto Ortopedico Rizzoli (IT)
- St Anna Children's Hospital (AT)
- National Cancer Institute (US)
- SickKids Foundation (CA)
- Dana-Farber/Boston Children's Cancer and Blood Disorders Center (US)
- St. Anna Children's Cancer Research Institute
- Medical University of Vienna (AT)
- Institut Curie (FR)
Publication Details
- Journal
- Molecular Cancer Therapeutics
- Published
- 2025-09-04
- DOI
- https://doi.org/10.1158/1535-7163.mct-25-0428
- Citations
- 1
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Sarcoma UK
- Alan B. Slifka Foundation
- National Institutes of Health
- National Cancer Institute