Exposure of electroporated Aiptasia larvae to Symbiodiniaceaen algae v1
This protocol describes how to expose larvae developed from electroporated zygotes 2 d post fertilization to the compatible Symbiodiniacaean algal strain SSB01 (Breviolum minutum) for 3 d, incubate them for three more days in algal-free medium and assess for establishment and/or maintenance of symbiosis afterwards. In addition, the protocol describes the optional precise assessment of survival.
Authors
- Christian CR Renicke (ORCID: https://orcid.org/0000-0003-3226-9840)
- Colin Henderson
- Natalie Swinhoe
- Emily K. Meier (ORCID: https://orcid.org/0000-0003-4293-8980)
Institutions
- Johns Hopkins University (US)
- Carnegie Institution for Science (US)
Publication Details
- Published
- 2025-05-24
- DOI
- https://doi.org/10.17504/protocols.io.8epv5oqw4g1b/v1
- Primary Topic
- Nematode management and characterization studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00