PSMtag - Anhydrous Labeling Protocol (50 µg Labeling) V2 v2

A water-free (anhydrous) method labeling peptides with PSMtag. Similar methods enable the use of PSMtag as low as 1 to 1 (wt/wt) peptide to tag ratios. What follows is a general protocol for labeling of an 50ug peptide sample using 75ug of PSMtag (1.5X wt/wt), allowing robust labeling efficiency of >98%. Labeling input can be scaled accordingly.