Study on the association of LINC00924 with prognosis and malignant progression of thyroid cancer

Abstract Objectives This study aimed to investigate the role of LINC00924 and its prognostic value in thyroid cancer (TC). Methods The study included cancer tissues from 101 TC patients and four TC cell lines. LINC00924 and miR-454-3p levels were measured using qRT-PCR. Patients were categorized into two groups according to LINC00924 levels. Cox regression analysis and Kaplan–Meier survival analysis were conducted to assess the correlation of LINC00924 with the pathological features of TC patients and its prognostic value. Additionally, validation of LINC00924 and miR-454-3p targeting relationship using dual luciferase reporter assay, and CCK-8 and Transwell assays were performed to evaluate TC cell proliferation, migration, and invasion. Results LINC00924 downregulated in TC tissues and cell lines, while miR-454-3p exhibited a marked upregulation. Lower LINC00924 expression correlated with advanced TNM stage, lymph node metastasis, and poorer overall survival. Cox analysis Kaplan-Meier survival analysis identified LINC00924 as an independent prognostic factor. Mechanistically, LINC00924 directly bound and negatively regulated miR-454-3p, acting as a molecular sponge. Functional experiments showed that LINC00924 suppressed TC cell proliferation, migration, and invasion, effects that were partially reversed by miR-454-3p. Conclusions LINC00924 may affect TC cell activity by regulating miR-454-3p. Furthermore, LINC00924 had potential as a clinical prognostic marker for TC.