Functionalized Graphene Quantum Dots for Thin-Film Illuminator and Cell Dyeing Applications

Graphene quantum dots (GQDs) have emerged as promising nanomaterials due to their unique optical properties, high biocompatibility, and tunable surface functionalities. In this work, GQDs were synthesized via a one-pot hydrothermal method and further functionalized using polyethylene glycol (PEG) of various molecular weights and sodium hydroxide to tailor their photoluminescence (PL) behavior and enhance their applicability in thin-film illumination and biological staining. PEG-modified GQDs exhibited a pronounced red-shift and intensified fluorescence response due to aggregation-induced emission, with GQD-PEG (molecular weight: 300,000) achieving up to eight-fold enhancement in PL intensity compared to pristine GQDs. The influence of solvent environments on PL behavior was studied, revealing solvent-dependent shifts and emission intensities. Transmission electron microscopy confirmed the formation of core–shell GQD clusters, while Raman spectroscopy suggested improved structural ordering upon modification. The prepared GQD thin films demonstrated robust fluorescence stability under prolonged water immersion, indicating strong adhesion to glass substrates. Furthermore, the modified GQDs effectively labeled E. coli, Gram-positive, and Gram-negative bacteria, with GQD-PEG and GQD-NaOH displaying red and green emissions, respectively, at optimal concentrations. This study highlights the potential of surface-functionalized GQDs as versatile materials for optoelectronic devices and fluorescence-based bioimaging.